Bacterial load of water used for livestock’s operations in Al Obied city of North Kordofan State, Sudan

Abstract

This study was conducted in Al Obied city in North Kordofan State in order to evaluate the bacterial load in water for livestock operations, based on bacteriological examinations and viable counts. A total of 40 water samples were obtained (10 from dairy farms, 14 from animal’s markets and 16 from slaughterhouses). All samples were cultured on Blood Agar and MacConkey for bacterial isolation and on nutrient agar for viable counts. The result of bacterial viable count of water samples collected from different sources was high and ranging between 7X108 and 36X108 CFU/ml. Application of analysis statistic using one way ANOVA for bacterial count in water collected from different sources, revealed that there was statistical significance (p. value= 0.000, p-value˂ 0.05) for water samples collected from different sites. Bacterial isolates from dairy farms comprised 10 isolates including 3 Escherichia coli (25.1%), 2 Klebsiella pneumoniae (16.7%), 2 Pseudomonas aerogenosa (16.7%), 1 Staphylococcus aureus (8.3%), 1 Streptococcus uberis (8.3%), 1 Bacillus subtilis (8.3%), 1 Micrococcus variens (8.3%) and 1 Micrococcus luteus (8.3%). Gram negative Bacterial isolates represented the higher percentage (58.5%) of the total bacterial isolates from dairy farms. Bacteria isolated from animals’ markets were 5 E. coli (31.3%), 4 Ps. aerogenosa (25.0%), 4 S. aureus (25.0%), 2 Str.uberis (12.5%), 1 B. subtilis (6.2%). Gram negative Bacteria represented the higher percentage (56.3%) of the total bacteria isolated from animals’ markets. Bacteria isolated from slaughterhouses were 5 S. aureus (31.3%), 4Ps. aerogenosa (25.0%), 3 Str. uberis (18.7%), 2 M. variens (12.5%),2 M. luteus (12.5%). Gram positive Bacteria represented the higher percentage (75.0%) of the total bacteria isolated from slaughterhouses.

 

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