Green method of synthesis silver nanoparticles as using fenugreek seeds extract (Trigonella foenum-graecum) and its application as antibacterial agent

Abstract

In the present research, spherical silver nanoparticles (AgNPs) of 33-75 nm size have been synthesized using AgNO3 solution and aqueous extract of Fenugreek plant seeds as a reducing agent. The principle is based on the reduction of AgNO3 by the extract of fenugreek seeds. The nanoparticles were characterized and investigated by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). The size and shape of the nanoparticles were found to be sensitive to the quantity of the extract. Silver nanoparticles are broad-spectrum antibacterial agents and the internalization of nanoparticles within cells could occur via processes including phagocytosis, fluid-phase endocytosis and receptor mediated endocytosis. This approach is not only of a green rapid synthesis kind and considered as a better alternative to chemical synthesis, but also found to be effective for large scale synthesis of silver nanoparticles.

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